- Gilda Mundson Farrell as Princess Daisy
- Johnny Farrell/Narrator as Mario
- Ballin Mundson as Luigi
- Det. Maurice Obregon as Yoshi
- Uncle Pio as Toad
- Casey as Bowser
- Capt. Delgado as Donkey Kong
- Gabe Evans as Diddy Kong
- German as Wario
- Thomas Langford as Waluigi
- Little man as Baby Mario
- Huerta as Bowser Jr.
- Maria as Princess Rosalina
- Anita Ellis, The singing voice of Rita Hayworth as Princess Peach
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