- Colonel Robert Gould Shaw as Mickey Mouse
- Private Silas Trip as Professor Ludwig Von Drake
- Sergeant Major John Rawlins as Goofy
- Major Cabot Forbes as Donald Duck
- Colonel James Montgomery as Chip
- Private Thomas Searles as Dale
- Private Jupiter Sharts as Scrooge McDuck
- Governor John Albion Andrew as Huey Duck
- Sergeant Major Mulcahy as Dewey Duck
- Captain Charles Fessenden Morse as Pete
- General Charles Garrison Harker as Louie Duck
- General George Crockett Strong as Casey Junior
- Frederick Douglass as Oswald the Lucky Rabbit
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