- Tramp (from Lady and the Tramp) as Mufasa
- Lady (from Lady and the Tramp) as Sarabi
- Benny as Young Simba
- Nanette as Young Nala
- Gnomeo as Adult Simba
- Juliet as Adult Nala
- Mario and Luigi (from Mario) as Timon and Pumbaa
- Shroom as Zazu
- Fishing Gnome as Rafiki
- Tybalt as Scar
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