- Mrs. Cermak as Clara Cluck
- Jill Conway as Minnie Mouse
- Sgt. Powers as Mickey Mouse
- Sharon Ames as Clarabelle Cow
- Det. Peter Hood as Ludwig Von Drake
- Billy as Pluto
- Det. Erica Lonsdale as Daisy Duck
- Lt. Ray Bozeman as Horace Horsecollar
- Nick Massey as Dewey Duck
- M. Miller as Huey Duck
- Trey as Louie Duck
- Henry Massey as Goofy
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