- Gerald O'Hara as Mickey Mouse
- Barbara O'Neil as Minnie Mouse
- Scarlett O'Hara as Daisy Duck
- Carreen O'Hara as Clarabelle Cow
- Suellen O'Hara as Ortensia
- Brent Tarleton as Donald Duck
- Stuart Tatleton as Pete
- Rhett O'Hara as Goofy
- Pork as Pluto
- and more
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