- Mr. Chips M.A. (Cantab.) as Mario
- Katherine as Princess Rosalina
- Dr. John Hamilton Wetherby D.D. (Cantab.) as Wario
- Max Staeffel M.A. (Oxon.) as Bowser
- John Colley, Peter Colley I, II and III as Waluigi, Toad, Yoshi, and Luigi
- Peter Colley as an adult as Kamek
- Sir John Colley as Boo
- Perkins as Toadsworth (uncredited)
- Mr. Jackson B.A. (Cantab.) as Oogtar
- Wainwright as Detective Herlock Solmes
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