• Mr. Chips M.A. (Cantab.) as Mario
  • Katherine as Princess Rosalina
  • Dr. John Hamilton Wetherby D.D. (Cantab.) as Wario
  • Max Staeffel M.A. (Oxon.) as Bowser
  • John Colley, Peter Colley I, II and III as Waluigi, Toad, Yoshi, and Luigi
  • Peter Colley as an adult as Kamek
  • Sir John Colley as Boo
  • Perkins as Toadsworth (uncredited)
  • Mr. Jackson B.A. (Cantab.) as Oogtar
  • Wainwright as Detective Herlock Solmes

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