- Mr. Chips M.A. (Cantab.) as Kermit the Frog
- Katherine as Janice
- Dr. John Hamilton Wetherby D.D. (Cantab.) as Dr. Teeth
- Max Staeffel M.A. (Oxon.) as Sam the Eagle
- John Colley, Peter Colley I, II and III as Lips, Floyd Pepper, Animal, and Beaker
- Peter Colley as an adult as Dr. Bunsen Honeydew
- Sir John Colley as Sweetums
- Perkins as Robin the Frog (uncredited)
- Mr. Jackson B.A. (Cantab.) as Fozzie Bear
- Wainwright as Rizzo the Rat
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