• Stan as Mario
  • Ollie as Luigi
  • Ginger Hammond as Princess Rosalina
  • Dan Forrester as Toadsworth
  • Sgt. Hippo as Toad
  • Colonel Ridley as Diddy Kong
  • Dr. Schickel as Bowser
  • Captain Baker as Donkey Kong
  • Aunt Martha as Princess Daisy
  • Aunt Agatha as Princess Peach
  • General Talylor as Wario
  • Doctor as Yoshi
  • Colonel Wayburn as Oogtar
  • General Burns as Bowser Jr.
  • Postman as Detective Herlock Solmes

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