- Tracy Turnblad as Princess Peach
- Penny Pingleton as Birdo
- Link Larkin as Mario
- Edna Turnblad as Princess Daisy
- Wilbur Turnblad as Diddy Kong
- Motormouth Maybelle as Toadette
- Seaweed Stubbs as Yoshi
- Little Inez as Princess Rosalina
- Amber Von Tussle as Wendy O. Koopa
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