• George Legenza as Mickey Mouse
  • Mary Simms as Minnie Mouse
  • Lee as Daisy Duck
  • Detective Sgt. Truscott and the film's narrator as Goofy
  • William B. 'Bill' Phillips as Donald Duck
  • Robert 'Bobby' Mais as Professor Ludwig Von Drake
  • Madeline Welton as Clarabelle Cow
  • Herbie Brooks as Oswald the Lucky Rabbit
  • Noyes Hinton - Gang Driver as Casey Junior

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