- George Legenza as Mickey Mouse
- Mary Simms as Minnie Mouse
- Lee as Daisy Duck
- Detective Sgt. Truscott and the film's narrator as Goofy
- William B. 'Bill' Phillips as Donald Duck
- Robert 'Bobby' Mais as Professor Ludwig Von Drake
- Madeline Welton as Clarabelle Cow
- Herbie Brooks as Oswald the Lucky Rabbit
- Noyes Hinton - Gang Driver as Casey Junior
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