- Twilight Sparkle (My Little Pony) as Red
- Granny Smith (My Little Pony) as Granny
- Tigger (Winnie the Pooh) as Wolf W. Wolf
- For Kirk the Woodsman, I Can't beside between... Tiger (An American Tale) or Once Ler (The Lorax)
- Tiger (An American Tale) will play Kirk the Woodsman and Once-Ler (The Lorax) is his extra
- Hammy the Squirrel (Over the Hedge) as Twitchy
- Jenner (NIMH) as Cheif Grizzly
- Sullivan (NIMH) as Bill Stork
- Spongebob as Nicky Flippers
- Rainbow Dash (My Little Pony) as Boingo
- Al (Toy Story 2) as Dolph
- Madame Medusa (The Rescuers) as Lesa
- Discord (My Little Pony) as Vincent
- Hinkle (Frosty the Snowman) as Keith
- Dodger (Oliver and Company) as Woolsworth
- Dodger's Gang (Oliver and Company) as the Sheep
- Mushu as Jimmy lizard
- Pain and Panic (Hercules) as Racoon Jerry
- Quick Draw McGraw as Japeth the Goat
- Boog (Open Season) as 2-Tone
- Su (Kung Fu Panda) as Zorra
- Frosty the Snowman as P*Biggie
- Hades (Hercules), Rats of NIMH (NIMH), Bikini Bottom Police (Spongebob), and Huns (Mulan) as Police
- Kessie (Pooh) as Quill
- What big teeth you had / Main Titles
- Against the Law / "Great Big World"
- Enter Rainbow Dash / Carrot Crumpets
- Twilight and Quill / "Critters Have Feelings"
- Rainbow's Cable Car / Encounter of Tigger
- Quick Draw McGraw / "Be Prepared"
- Tigger's Story
- Tigger and Rainbow Dash
- Hammy the Squirrell
- Tiger's story / "The Schnitzel Song" / Dash confronts Tiger
- Tiger chops down trees
- Granny Smith's sking contest
- "Twilight is Blue"
- Twilight is Gone
- Bad Pegasus Rainbow Dash
- "Top of the woods"
- The Rescue
- The chase / End of the Line / Rainbow, Al, Medusa, Discord, and Hinkle's desmie
- Goodies are Eaten
- The Happily Ever After Agency
- End Credits
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