- Roy A. Eberhardt as Mickey Mouse
- Beatrice "The Bear" Leep as Minnie Mouse
- Napoleon Bridger "Mullet Fingers" Leep as Professor Ludwig Von Drake
- Officer David Delinko as Donald Duck
- "Curly" Brannit as Goofy
- Mr. Eberhardt as Casey Junior
- Mrs. Eberhardt as Daisy Duck
- Chuck Muckle as Mortimer Mouse
- Kimberly as Clarabelle Cow
- Garrett as Dale
- Mayor Grandy as Pete
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