- Alan Spender as Mickey Mouse
- Victoria Kowelska as Clarabelle Cow
- Major Marc Bennett as Ludwig Von Drake
- Margaret as Minnie Mouse
- Christopher as Pluto
- Dr. Burkhardt as Scrooge McDuck
- Joseph C. Callahan as Donald Duck
- Mr. Whitmore as Pete
- Mrs. Whitmore as Daisy Duck
- Tony as Goofy
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