• Huckleberry Finn as Mario
  • Jim as Luigi
  • The King as Toad
  • The Duke as Yoshi
  • Col. Grangerford as Wario
  • Pap as Donkey Kong
  • Mrs. Loftus as Princess Peach
  • Widow Douglas as Princess Daisy
  • Maryjane Wilks as Princess Rosalina
  • Susan Wilks as Birdo
  • Miss Watson as Toadette
  • Jim's Wife as Wendy O. Koopa

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