- Charlotte Hollis as Birdo
- Miriam Deering as Princess Peach
- Dr. Drew Bayliss as Mario
- Velma Cruther as Princess Daisy
- Harry Willis as Luigi
- Big Sam Hollis as Toad
- Jewel Mayhew as Toadette
- John Mayhew as Wario
- Foreman as Waluigi
- Town Gossip as Princess Rosalina
- Reporter as Yoshi
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