- Lieutenant Phil Gaines as Mario
- Nicole Britton as Pauline
- Marty Hollinger as Luigi
- Sergeant Louis Belgrave as Toad
- Paula Hollinger as Princess Peach
- Leo Sellers as Donkey Kong
- Santuro as Yoshi
- Herbie Dalitz as Oogtar
- Gloria Hollinger as Princess Daisy
- Bus Driver as Bowser Jr.
- Bellamy as Detective Herlock Solmes
- Peggy Summers as Princess Rosalina
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