• Barbara Graham as Princess Peach
  • Edward S. Montgomery as Mario
  • Peg as Princess Daisy
  • Carl G.G. Palmberg as Luigi
  • Henry L. Graham as Yoshi
  • Emmett Perkins as Toadsworth
  • John R. Santo as Diddy Kong
  • Bruce King as Oogtar
  • District Attorney Milton as Donkey Kong
  • Attorney Richard G. Tibrow as Wario
  • Al Matthews as Mallow
  • Father Devers as Mighty Plumber
  • San Quentin Warden as Toad
  • Barbara as Birdo
  • and more

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