• Manny as Alex
  • Diego as Marty
  • Macrauchenia as Melman
  • Granny as Gloria
  • Sid as King Julien
  • Aardvark as Maurice
  • Scrat as Mort (both really small)
  • Elmo (from Sesame Street) and Fievel (from An American Tail) as Mason and Phil
  • Big Bird (from Sesame Street) as Kowalski
  • Leo (from Little Einsteins) as Skipper
  • Danny (from CDD) as Rico
  • Boo Boo Bear (from Yogi Bear) as Private
  • Scratte as Marlene

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