- Tigger (from Winnie the Pooh) as Manny
- Elmo (from Sesame Street) as Sid
- Grover (from Sesame Street) as Diego
- Binky Barnes (from Arthur) as Scrat
- Tiggress (from Kung Fu Panda) as Ellie
- Bert and Ernie (from Sesame Street) as Crash and Eddie
- Baby Natasha (from Sesame Street) as Baby Pechaes
- Humphrey (from Sesame Street) as Buck
- Rudy as himself
- The Guanlongs as himself
- The Quetzalcoatlus as himself
- Grundgetta (from Sesame Street) as Scratty
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