- Indiana Jones as Mario
- Marion as Princess Rosalina
- Short Round as Luigi
- Irina Spalko as Princess Peach
- Rene Belloq as Bowser
- Sallah as Donkey Kong
- Mutt Williams as Wario
- Walter Donovan as Waluigi
- Colonel Dietrich and Arnold Toht as Yoshi and Diddy Kong
- Mola Ram as Toad
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