- Johnny Knoxville as Kermit the Frog
- Steve-O as Sam the Eagle
- Chris Pontius as Dr. Bunsen Honeydew
- Dave England as Scooter
- Bam Margera as Beaker
- Preston Lacy as Robin the Frog
- Ryan Dunn as Crazy Harry
- Ehren McGhehey as Dr. Teeth
- Wee-Man as Animal
- Brandon DiCamillo as Rizzo the Rat
- Chris Rabb as Wayne
- and more
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