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- Jay Jay as Megamind
- Snuffy as Minion
- Big Jake as Metro Man
- Ripslinger (from Planes) as Tighten
- Tracy as Roxanne
- Budgie (from Budgie) as Space Dad
- Herky as Bernard
- Oliver (from Thomas) as Prison
- Thomas and Percy (from Thomas) as Prison Guards
- TUGS Characters as Minions