- Jiminy Glick/David Lynch as Mario
- Dixie Glick as Princess Rosalina
- Natalie Coolidge as Princess Peach
- Dee Dee as Princess Daisy
- Andre Devine as Luigi
- Miranda Coolidge as Birdo
- Haygood Lewkin as Toad
- Sharon as Toadette
- Barry King as Yoshi
- Ben DiCarlo as Oogtar
- Gunnar "MC GUN" Jorge as Wario
- Jay Schiffer as Waluigi
- Randall Bookerton as Donkey Kong
- Matthew Glick as Diddy Kong
- Bellhop/Barber as Bowser
- Tibor as Bowser Jr.
- June as Sultan Pasbah
- Johnny Stompanato as Waluigi
- and more
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