- Jonah Hex as Mickey Mouse
- Quentin Turnbull as Pete
- Lilah as Minnie Mouse
- Cassie as Daisy Duck
- Doctor Cross Williams as Pluto
- Lieutenant Grass as Jiminy Cricket
- Ulysees S. Grant as Chip
- Smith as Dale
- Lieutenant Evan as Donald Duck
- Colonel Slocum as Oswald the Lucky Rabbit
- Adleman Lusk as Ludwig Von Drake
- Jeb Turnbull as Huey Duck
- Brent Hinds as Scrooge McDuck (cameo)
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