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- Spongebob Squarepants as Roger Rabbit
- Sandy Cheeks as Jessica Rabbit
- Ben Tennyson as Gorilla
- Numbuh 4 as Eddie V.
- Jenny Wakeman as Dolores
- Gaz as R.K. Maroon
- Static Shock as Mickey Mouse
- Numbuh 4 as Donald Duck
- Numbuh 2 as Goofy
- Jimmy Neutron's Rocket as Benny the Cab
- Chowder as Woody Woodpecker
- Trixie Tang as Tweety
- Timmy Turner as Shoe
- Cosmo as Bugs Bunny
- Diddy Kong as Daffy Duck
- Billy (Billy and Mandy) as Porky Pig
- Donkey Kong as Sylvester
- Doidle as The Bad Wolf
- Plankton as Judge Doom
- Mario, Luigi, and Toad as Weasels