- Mike Hammer as Mickey Mouse
- Dr. G.E. Soberin as Donald Duck
- Carl Evello as Huey Duck
- Eddie Yeager as Ludwig Von Drake
- Lt. Pat Murphy as Minnie Mouse
- Friday as Daisy Duck
- Velda as Clarabelle Cow
- Christina Bailey as Oretensia
- Nick as Pluto
- Charlie Max as Goofy
- Doc Kennedy as Pete
- Police Detective as Mortimer Mouse
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