• Jason as Scooby Doo
  • Great Bear's Son as Shaggy
  • Chander as Fred
  • Lua as Daphne
  • Pachycepalosaurus Mother as Velma
  • Great Bear as Alejo Otero
  • Kong as Luis Otero
  • Susanna as Sofia Otero
  • Dr. Lorna Jenkins as Dona Delores
  • Amina as Chiquita
  • Wiseman as Diego Fuente
  • Eric Tann Tannenbaum as El Curandero
  • Chiros as El Chupacabra
  • Queen Reptilia as Museum Guide
  • Tiger Lucy as Charlene
  • Omar as Paco
  • Giggles as Mr. Smiley
  • Chinese Dragon as Ghost of Senor Otero
  • Billy as Jorge Otero

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