Cast
- Jason as Scooby Doo
- Great Bear's Son as Shaggy
- Chander as Fred
- Lua as Daphne
- Pachycepalosaurus Mother as Velma
- Great Bear as Alejo Otero
- Kong as Luis Otero
- Susanna as Sofia Otero
- Dr. Lorna Jenkins as Dona Delores
- Amina as Chiquita
- Wiseman as Diego Fuente
- Eric Tann Tannenbaum as El Curandero
- Chiros as El Chupacabra
- Queen Reptilia as Museum Guide
- Tiger Lucy as Charlene
- Omar as Paco
- Giggles as Mr. Smiley
- Chinese Dragon as Ghost of Senor Otero
- Billy as Jorge Otero