- Larry as Thomas
- Walt as Edward
- Cecil Trewilliger (from The Simpsons) as Henry
- Krusty the Clown (from The Simpsons) as Gordon
- Kirk Von Houten (from The Simpsons) as James
- Igner as Percy
- Poochie (from The Simpsons) as Toby
- Big Mickey (from TUGS) as Duck
- Carl (from The Simpsons) as Donald
- Lenny (from The Simpsons) as Douglas
- Old Gil (from The Simpsons) as Oliver
- Superintendent Chlamers (from The Simpsons) as Diesel
- Todd Flaners (from The Simpsons) as Bill
- Rodd Flaners (from The Simpsons) as Bill
- Sideshow Mel (from The Simpsons) as BoCo
- Agnes Skinner (from The Simpsons) as Daisy
- Jacqueline Bouvier (from The Simpsons) as Mavis
- Itchy (from The Simpsons) as Stepney
- Helen Lovejoy (from The Simpsons) as Emily
- Dr. Hibbert (from The Simpsons) as Bertie
- Dr. Marvin Monroe (from The Simpsons) as Salty
- Troy McClure (from The Simpsons) as Harvey
- The Sea Captain (from The Simpsons) as Fergus
- Rock Brother 1 (from Theodore Tugboat) as Skarloey
- Rock Brother 3 (from Theodore Tugboat) as Rheneas
- Big Mickey (from TUGS) as Sir Handel
- Oliver (from Theodore Tugboat) as Peter Sam
- Dartmouth (from Theodore Tugboat) as Rusty
- Stewiacke (from Theodore Tugboat) as Duncan
- Rock Brother 2 (from Theodore Tugboat) as Duke
- Clayton (from Theodore Tugboat) as Freddie
- Eddie (from TUGS) as Mighty
- Frank (from TUGS) as Mac
- Tim (from The Cleveland Show) as Arthur
- Lisa (from The Simpsons) as Lady
- Frank Grimes (from The Simpsons) as Diesel 10
- Legs (from The Simpsons) as Splatter
- Louie (from The Simpsons) as Dodge
- Lou (from The Simpsons) as Terence
- Eddie (from The Simpsons) as Trevor
- Barry (from American Dad) as Toad
- Moe (from The Simpsons) as Derek
- Frank Grimes Jr. (from The Simpsons) as Scruffey
- Enda Crabapple (from The Simpsons) as Elizabeth
- Groundskeeper Willie (from The Simpsons) as George
- Mr. Burns (from The Simpsons) as Spencer
- Marge (from The Simpsons) as Caroline
- Principal Skinner (from The Simpsons) as Cranky
- Comic Book Guy (from The Simpsons) as Scruffey
- The Pixie (from Skipper & Skeeto: The Midnight Mystery) as Mr. Percival
- Artie Pye (from The Simpsons) as Harold
- Maggie (from The Simpsons) as Rosie
- Bluenose (from TUGS) as Smudger
- Johnny Tightlips (from The Simpsons) as Bulstrode
- Steve (from American Dad) as Stanley
- Snake Jailbird (from The Simpsons) as The Spiteful Brakevan
- Lorry 1 (from TTTE) as Jack
- Lorry 3 (from TTTE) as Alfie
- Freddie (from TTTE) as Oliver (Pack)
- The Foreign Engine (from RWS) as Max
- Mallard (from RWS) as Monty
- Butch (from TTTE) as Kelly
- Cranky (from TTTE) as Byron
- Smudger (from TTTE) as Ned
- Caroline (from TTTE) as Isobella
- Lorry 2 (from TTTE) as Nelson
- Derek (from TTTE) as Patrick
- Colin (from TTTE) as Buster
- The Refreshment Lady (from TTTE) as MIss Jenny
- Tom Tipper (from TTTE) as The Foreman
- Homer (from The Simpsons) as Lorry 1
- Ned Flanders (from The Simpsons) as Lorry 2
- Bart (from The Simpsons) as Lorry 3
- Artie Ziff (from The Simpsons) as D261
- Database (from The Simpsons) as Whiff
- Millhouse Von Houten (from The Simsons) as Scruff
- Lester (from The Cleveland Show) as Dennis
- Rallo (from The Cleveland Show) as Porter
- Barry (from Ametican Dad) as Samson
- George (from Theodore Tugboat) as Timothy
- Dewy Largo (from The Simpsons) as Gator
- Dr. J. Loren Pyror (from The Simpsons) as Reg
- Shelby's Father (from The Simpsons) as Neville
- Russ Cargill (from The Simpsons) as Hector
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Larry (Futurama)/Thomas
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