- Daniel Rafferty as Mario
- Audrey Woods as Princess Peach
- Serena Jamison as Lady Lima
- Thorne Jamison as Toadsworth
- Sara Woods as Birdo
- Judge Abramovitz as Yoshi
- Michael Rawson as Toad
- Adamo Shandela as Wario
- Ashton Phelps as Bowser Jr.
- Leslie as Princess Daisy
- Gary Gadet's assistant as Princess Rosalina
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