- Elle Woods as Princess Peach
- Emmett Richmond as Mario
- Warner Huntington III as Luigi
- Vivian Kensington as Birdo
- Professor Stromwell as Yoshi
- Professor Callahan as Bowser
- Paulette Bonafonte as Princess Daisy
- Brooke Taylor-Windham as Princess Rosalina
- Margot and Serena as Baby Princess Peach and Baby Princess Daisy
- David Kidney as Donkey Kong
- Chutney Windman as Dixie Kong
- Mrs. Windham-Vandermark as Toadette
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