- Gabriel "Gabe" Burton as Mickey Mouse
- Rosemary Telesco as Minnie Mouse
- Adam Burton and Leslie Burton as Professor Ludwig Von Drake and Ortensia
- Ralph as Oswald the Lucky Rabbit
- Birdie as Daisy Duck
- Ronny as Goofy
- Mickey and Jackie Telesco as Pluto and Clarabelle Cow
- Sam as Donald Duck
- Tim Staples as Chip
- Mike Chat as Dale
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