- Josephine "Jo" March as Lanolin
- Friedrich Bhaer as Orson
- Margaret "Meg" March as Cloe
- Amy March as Arlene
- Elizabeth "Beth" March as Penelope Pussycat
- Theodore "Laurie" Laurence as Bo
- John Brooke as Edward R. Furrow
- Mr. Laurence as Roy
- Aunt March as Lenore
- Margaret "Marmee" March as Liz Wilson
- Mr. March as Garfield
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