- Josephine "Jo" March as Princess Peach
- Friedrich Bhaer as Mario
- Margaret "Meg" March as Princess Daisy
- Amy March as Toadette
- Elizabeth "Beth" March as Princess Rosalina
- Theodore "Laurie" Laurence as Luigi
- John Brooke as Toad
- Mr. Laurence as Oogtar
- Aunt March as Birdo
- Mr. March as Donkey Kong
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