• Josephine "Jo" March as Princess Peach
  • Friedrich Bhaer as Mario
  • Margaret "Meg" March as Princess Daisy
  • Amy March as Toadette
  • Elizabeth "Beth" March as Princess Rosalina
  • Theodore "Laurie" Laurence as Luigi
  • John Brooke as Toad
  • Mr. Laurence as Oogtar
  • Aunt March as Birdo
  • Mr. March as Donkey Kong

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