- Josephine "Jo" March as Miss Piggy
- Friedrich Bhaer as Kermit the Frog
- Margaret "Meg" March as Annie Sue Pig
- Amy March as Janice
- Elizabeth "Beth" March as Wanda
- Theodore "Laurie" Laurence as Gonzo the Great
- John Brooke as Dr. Bunsen Honeydew
- Mr. Laurence as Floyd Pepper
- Aunt March as Miss Mousey
- Mr. March as Sam the Eagle
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