- Josephine "Jo" March as Rosita
- Friedrich Bhaer as Big Bird
- Margaret "Meg" March as Abby Cadabby
- Amy March as Zoe
- Elizabeth "Beth" March as Maria
- Theodore "Laurie" Laurence as Elmo
- John Brooke as Ernie
- Mr. Laurence as Grover
- Aunt March as Lulu
- Margaret "Marmee" March as Susan
- Mr. March as Cookie Monster
- Hannah as Gina
- Sally Moffat as Gabi
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