- Ace Bunny as Adult Simba
- Lexi Bunny as Adult Nala
- Melvin the Martian as Scar
- Tech E.Coyote as Timon
- Slam as Pumbaa
- Buster Bunny(from Tiny Toon Adventures) as Young Simba
- Babs Bunny(from Tiny Toon Adventures) as Young Nala
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