- Bugs Bunny as Peanut Otter
- Tweety as Baby Butter Otter
- Lola Bunny as Jelly Otter
- Road Runner as Munchy Beaver
- Daffy Duck as Flick Duck
- Penelope Pussycat as Pinch Raccoon
- Porky Pig as Scootch Raccoon
- Granny as Opal Otter
- Goldfish as Ernest Otter
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