- Nick Cochran as Mickey Mouse
- Julie Benson as Daisy Duck
- Lawrence C. Trumble as Professor Ludwig Von Drake
- Lt. Sebastian as Horace Horsecollar
- Margie as Minnie Mouse
- Vincent Halloran as Oswald the Lucky Rabbit
- Martin Stewart as Mortimer Mouse
- Itzumi as Huey Duck
- Kwan Sum Tang as Pluto
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