• Donna Carmichael (née Sheridan) as Princess Daisy
  • Sophie Sheridan as Princess Peach
  • Sam Carmichael as Mario
  • Harry Bright as Luigi
  • Bill Anderson as Toad
  • Sky as Donkey Kong
  • Rosie Mulligan as Princess Rosalina
  • Tanya Chesham-Leigh as Birdo
  • Pepper as Wario
  • Petros as Waluigi
  • Ali as Wendy O. Koopa
  • Lisa as Toadette
  • Gregoris as Bowser
  • Father Alex as Bowser Jr.
  • Piano Player as Yoshi (cameo)
  • Greek God as Boo (cameo)
  • Greek Goddess as Toadiko (cameo)

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