- Donna Carmichael (née Sheridan) as Princess Daisy
- Sophie Sheridan as Princess Peach
- Sam Carmichael as Mario
- Harry Bright as Luigi
- Bill Anderson as Toad
- Sky as Donkey Kong
- Rosie Mulligan as Princess Rosalina
- Tanya Chesham-Leigh as Birdo
- Pepper as Wario
- Petros as Waluigi
- Ali as Wendy O. Koopa
- Lisa as Toadette
- Gregoris as Bowser
- Father Alex as Bowser Jr.
- Piano Player as Yoshi (cameo)
- Greek God as Boo (cameo)
- Greek Goddess as Toadiko (cameo)
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