• Mario as Tony Stark/Iron Man
  • Cranky Kong as Steve Rogers/Captain America
  • Toadsworth as Dr. Bruce Banner
  • Donkey Kong as The voice of Hulk
  • Geno as Thor
  • Wrinkly Kong as Natasha Romanoff/Black Widow
  • Diddy Kong as Clint Barton/Hawkeye
  • Princess Daisy as Maria Hill
  • Mallow as Nick Fury
  • Bowser as Wario
  • Wendy O. Koopa as Wanda Maximoff/Scarlet Witch
  • King K.Rool as James Rhodes/War Machine
  • Troff as Baron Strucker
  • Birdo as Peggy Carter
  • Roy Koopa as Heimdall
  • Pauline as Laura Barton
  • Scoff as Thanos
  • Larry Koopa as Erik Selvig
  • Yoshi as World War ll Veteran
  • Luigi as Ulysses Klaw
  • Toad as Dr. Helen Cho
  • Yellow Toad as Doctor List
  • Princess Peach as Madame B.

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