- Mario as Barney
- Peach as Baby Bop
- Luigi as BJ
- Baby Mario as Cody Newton
- Toadette as Abby Newton
- Birdo as Marcella
- Donkey Kong as Grandpa Greenfield
- Candy Kong as Grandma Greenfield
- Rosalina as Ms. Mildred Goldfinch
- Waluigi as Mr. Millet
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