• Mario as Bobby
  • Yoshi as Derek
  • Birdo as Kelly
  • Baby Mario as Jake
  • Baby Luigi as Al
  • Mallow as Roger
  • Toad as Uncle Ted
  • Toadette as Aunt Ruth
  • Luigi as Howard
  • Princess Daisy as Martha
  • Shy Guy as Webley
  • Oogtar as George
  • Diddy Kong as Jeffery
  • Lanky Kong as Jess
  • Princess Peach as Jackie
  • Candy Kong as Miss Graves
  • Donkey Kong as Captain Squash
  • Humba Wumba (From Banjo-Kazooie) as Charlotte
  • Troff as Meeker
  • Scoff as Snurd
  • Cranky Kong as Mr. Generic
  • Wario as Markie (both are rivals to Mario and Bobby)
  • Wendy "Kootie Pie" O. Koopa as Mrs. Hula
  • Larry "Cheatsy" Koopa as Gordon Hula
  • King K. Rool as Alien
  • Kaptain Skurvy as Crab
  • Bowser as Monster

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