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- Mario as Earthworm Jim
- Yoshi as Peter Puppy
- Princess Peach as Princess What's-Her-Name
- Wendy "Kootie Pie" O. Koopa" as Queen Slug-For-a-Butt
- Bowser as Psycrow
- Ludwig "Kooky" Von Koopa as Professor Monkey-For-a-Head
- Smithy as Evil the Cat
- Koopas and Goombas as Henchrats
- Morton "Big Mouth" Koopa Jr. as Evil Jim
MORE COMING SOON