- Mario as Edward Elric
- Yoshi as Alphonse Elric
- Luigi as Roy Mustang
- Donkey Kong as Shou Tucker
- Princess Peach as Winry Rockbell
- Princess Daisy as Riza Hawkeye
- Birdo as Izumi Curtis
- Bowser as Scar
- Iggy and Lenny Koopa as Greed and King Bradley
- Smithy as Father
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