- Mario as Indiana Jones
- Toadsworth as Henry Jones Sr.
- Princess Peach as Marion Ravenwood
- Princess Daisy as Willie Scott
- Yoshi as Short Round
- Luigi as Sallah
- Pauline as Elsa Schneider
- Wendy O Koopa as Irina Spalko
- Wario as Rene Belloq
- Ludwig Kooky Von Koopa as Arnold Toht
- Bowser as Mola Ram
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