- Mario as CJ
- Yoshi as Botley
- Luigi as Edison
- Toad as Frankie
- Mallow as the Octopus
- Donkey Kong as the Caterpillar
- Princess Peach as Polly
- Wendy O Koopa as Mrs. Flores
- Uncle Chuck (from Sonic) as the Rabbit
- Birdo as Kisha
- Princess Daisy as Eleanor
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