• Mario as Captain Kirk
  • Luigi as Spock
  • Yoshi as Dr. McCoy
  • Toad as Scotty
  • Donkey Kong as Sulu
  • Funky Kong as Chekov
  • Princess Peach as Ulhur
  • Bowser as the Gorn
  • Waluigi as Mirror Spock
  • Petey Pirhana as Khan Noonen Sing

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