- Mario as Baloo
- Luigi as Bagheera
- Peach as Mowgli's Mother
- Toadsworth as Mowgli's Father
- Yoshi as Kaa
- Toad as Mowgli
- Toadette as Shanti
- Bowser as Shere Khan
- Donkey Kong as Colonel Hathi
- Wario as Louie
- Baby Mario as Baby Mowgli
- Tails (from Sonic) as Ranjan
- Link (from Zelda) as Buzzie
- First part - Opening credits
- Second part - Luigi Discovers Toad/Toad's Jungle Life
- Third part - Toad and Luigi encounter Yoshi
- Fourth part - "Donkey Kong's March"
- Fifth part - Luigi and Toad's Argument
- Sixth part - Toad meets Mario/"The Bare Necessities"
- Seventh part - Toad meets Wario/"I Wanna be like You"
- Eighth part - Luigi and Mario's Moonlight Talk
- Ninth part - Toad Runs away
- Tenth part - Bowser Arrives/"Donkey Kong's March reprise"
- Eleventh part - Toad and Yoshi the second encounter/"Trust in Me"
- Twelfth part - Yoshi and Bowser's conversation
- Thirteenth part - Toad's friends/"That's what friends are for"
- Fourteenth part - Toad meets Bowser/Bowser Fights
- Fifth part - The Grand Finale/"My Own Home"
- Sixteenth part - The Bare Necessities Reprise
- Seventeenth part - Ending credits
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