• Mario as Jago
  • Luigi as TJ Combo
  • Toad as Chief Thunder
  • Dry Bones as Spinal
  • Donkey Kong as Sabrewulf 
  • Bowser as Fulgore
  • Boshi as Riptor 
  • Peach as B. Orchid 
  • Wario as Glacius
  • Waluigi as Cinder 
  • Toadsworth as Director 
  • Oogtar as Robert
  • Pauline as Gracie
  • Funky Kong as Surgeon 
  • Nurse Peach as Nurse 
  • Yoshi as Oliver the Horse 
  • Dr. Mario as Doctor 
  • Lanky Kong as Tiger Spirit 
  • Chunky Kong as Announcer
  • Mallow as Count Von Sabrewulf 
  • Diddy Kong as B. Eagle 
  • Bowser's Minions as Fulgores 
  • King K. Rool as Eyedol 
  • Kremlings as Police Guards 

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